5 latest biotechnology breakthroughs, from genome sequencing to microbiome manipulation, revolutionizing medicine, ...

recombinant DNA technology / DNA cloning; gene cloning; cloning A technology that uses enzymes to cut and paste together DNA sequences of interest.

Cloning a gene between two restriction sites, and the expression of a protein, are almost always taken for granted—but a frequently overlooked detail is that sometimes, as a promoter is placed ...

Historically, figuring out what a gene does by cloning its DNA and expressing its protein was done one gene at a time. The new technology simultaneously clones and expresses thousands of ...

Summary Among the vital tools in this field are cloning enzyme services, which facilitate the cloning of DNA, and the generation of homology models, essential for understanding protein structures and ...

Cell-Free DNA Cloning Cell-based methods for cloning DNA that rely on E. coli are time-consuming and inefficient. OriCiro has developed new DNA cloning techniques that bypass E. coli completely ...

A map of DNA methylation changes in human organs could help researchers to discover more targets for anti-ageing therapies.

Clyde A. Hutchison III, Hamilton O. Smith, Cynthia Pfannkoch, J. Craig Venter, Cell-Free Cloning Using ϕ29 DNA Polymerase, Proceedings of the National Academy of Sciences of the United States of ...

This cloning-free method involves the insertion of long DNA fragments in two steps. In the first step, a small portion (less than 200 nucleotides) of the long fragment is inserted in the genome.

The black-footed ferret was believed extinct until 18 were discovered on a Wyoming ranch. Now scientists have cloned one using 33-year-old DNA.