本研究提出了一种基于错配 crRNA 的 CRISPR/Cas12a 检测平台，实现了对 kelch13 基因 Q613、Q613E 和 Q613H ...

CRISPR screening is a cost‑effective, and potentially cost-saving, approach, because it derisks drug development. However, developing a screening platform and recruiting people with the ...

CRISPR libraries offer huge advantages for high-throughput screening, including unprecedented editing efficiency, accuracy, and speed. This article walks through the fundamentals of CRISPR screening ...

CRISPR screening has become of great importance for drug target discovery, particularly in oncology, because we can now address questions like finding biomarkers for patient stratification and new ...

How CRISPR Is Revolutionizing Screening Technology CRISPR facilitates the creation of knockout, interference, and activation screens that precisely and comprehensively interrogate genomic function ...

There’s more to CRISPR screening than knockouts. There’s inhibition and activation—and multiplexing, too. Besides being more nuanced, the new approaches can reach once-inaccessible genomic ...

Yicheng Wang, Anant K. Menon, Yuta Maki, Yi-Shi Liu, Yugo Iwasaki, Morihisa Fujita, Paula A. Guerrero, Daniel Varó’n Silva, Peter H. Seeberger, Yoshiko Murakami, Taroh Kinoshita, Genome-wide CRISPR ...

Researchers combine pooled CRISPR screening with single-cell RNA sequencing to enable high-resolution analysis of transcriptional profiles on a large scale. Diagram of CRISPR-seq (4). Genetic screens ...

CRISPR screens in primary T lymphocytes have proved challenging, owing to complex issues around the introduction of the screening components and Cas9 in particular. Horizon has adapted its established ...

CRISPR screen identifies new anti-inflammatory drug target Date: November 11, 2021 Source: Vanderbilt University Medical Center Summary: A metabolic enzyme that has been studied in cancer biology ...

Using a genomewide CRISPR screen, the Vanderbilt team discovered that deleting a protein made by the gene TRAF3 causes cells to proliferate without stopping, even after they reach a certain ...